Inhalation therapy with M1 inhibits experimental melanoma development and metastases in mice

Lucas Ferrari de Andrade; Brian Mozeleski; Aline Raquell Leck; Gustavo Rossi; Cleber Rafael Vieira da Costa; Fernando de Souza Fonseca Guimarães; Rafael Zotz; Katia Fialho do Nascimento; Carolina Camargo de Oliveira; Dorly de Freitas Buchi; Edvaldo da Silva Trindade

doi:10.1016/j.homp.2015.08.007

Homeopathy, Table of Contents

Homeopathy 2016; 105(01): 109-118
DOI: 10.1016/j.homp.2015.08.007

Original Paper

Inhalation therapy with M1 inhibits experimental melanoma development and metastases in mice

Authors

Lucas Ferrari de Andrade

¹Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Biologia Celular, Laboratório de Células Inflamatórias e Neoplásicas, Coronel Francisco Heráclito dos Santos, Curitiba, PR, 81530-900, Brazil
Brian Mozeleski

²Institut Pasteur, Unité de Régulation Immunitaire et Vaccinologie, Département d'Immunologie, 25-28 rue du Docteur Roux, 75724, Paris, France
Aline Raquell Leck

¹Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Biologia Celular, Laboratório de Células Inflamatórias e Neoplásicas, Coronel Francisco Heráclito dos Santos, Curitiba, PR, 81530-900, Brazil
Gustavo Rossi

¹Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Biologia Celular, Laboratório de Células Inflamatórias e Neoplásicas, Coronel Francisco Heráclito dos Santos, Curitiba, PR, 81530-900, Brazil
Cleber Rafael Vieira da Costa

¹Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Biologia Celular, Laboratório de Células Inflamatórias e Neoplásicas, Coronel Francisco Heráclito dos Santos, Curitiba, PR, 81530-900, Brazil
Fernando de Souza Fonseca Guimarães

³Queensland Institute of Medical Research, Immunology in Cancer and Infection, 300 Herston Rd, Brisbane, QLD, 4006, Australia
Rafael Zotz

⁴Pontifícia Universidade Católica do Paraná, Biotério Central, 155, Imaculada Conceição, Bairro Prado Velho, Curitiba, PR, 80215-901, Brazil
Katia Fialho do Nascimento

¹Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Biologia Celular, Laboratório de Células Inflamatórias e Neoplásicas, Coronel Francisco Heráclito dos Santos, Curitiba, PR, 81530-900, Brazil
Carolina Camargo de Oliveira

¹Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Biologia Celular, Laboratório de Células Inflamatórias e Neoplásicas, Coronel Francisco Heráclito dos Santos, Curitiba, PR, 81530-900, Brazil
Dorly de Freitas Buchi

¹Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Biologia Celular, Laboratório de Células Inflamatórias e Neoplásicas, Coronel Francisco Heráclito dos Santos, Curitiba, PR, 81530-900, Brazil
Edvaldo da Silva Trindade

¹Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Biologia Celular, Laboratório de Células Inflamatórias e Neoplásicas, Coronel Francisco Heráclito dos Santos, Curitiba, PR, 81530-900, Brazil

Subject Editor:

Abstract

Background: M1 is a homeopathic medicine with immunostimulatory properties used mainly by cancer patients to complement current therapies. Metastatic melanoma is a skin-originated form of cancer without a single therapy able to produce high rate and sustained responses, which attracts the use of complementary therapies such as M1. However, M1's anti-melanoma effects remain to be pre-clinically demonstrated. Therefore in the present work, we utilized a pulmonary metastatic melanoma model and a subcutaneous melanoma growth model to investigate the potential benefits of treatment with M1.

Methods: C57BL/6 mice were injected intravenously or subcutaneously with B16F10 mouse melanoma cells. After 24 h, mice were treated with either M1 or vehicle (water) for 14 days, euthanized and harvested for multi-parameter pulmonary and tumor analyses.

Results: Mice treated with M1 had significantly lower tumor burden in the lungs and subcutaneous tissue than control mice. Furthermore, tumors were impaired in proliferation and tumor related angiogenesis by the inhibition of myeloid derived suppressor cells (MDSC) positive for angiotensin II type 1 receptor (AT1R).

Conclusion: Altogether these data suggest M1 is an efficient candidate for melanoma therapy to be considered for future clinic studies as this study is the first supporting the idea that melanoma patients may benefit with the treatment. The treatment with M1 provides advantages considering the highly-diluted properties and a cost effective alternative to costly chemotherapeutic approaches with, if any, lower toxicity.

Keywords

Melanoma - Homeopathy - M1 - Angiotensin II receptor - Angiogenesis - Tumor growth

Full Text

References

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